Phage isolation

Bacillus phage isolation

This project had multiple objectives. On the one hand, we aimed to find out whether the viral diversity associated mainly with Bacillus subtilis is greater than previously known and assumed. In doing so, we had both the organismal and genomic diversity in mind. On the other hand, it enabled us to isolate our own phage strains which we could use as model systems for further studies.

Status: This project was usually addressed within the framework of student work, such as Bachelor and Master Theses. At the moment, we do not plan to further isolate phages. However, we acquired many strains during previous attempts, which need to be characterised and published. If you are interested in such a project, please contact us.

Associated publications

Baena Lozada LP, Hoppert M, Hertel R (2020) Phage vB_BmeM-Goe8 infecting Bacillus megaterium DSM319. Arch Virol 165:515–517 (DOI:10.1007/s00705-019-04513-5).

Furrer AD, Bömeke M, Hoppert M, Hertel R (2020) Phage vB_BveM-Goe7 represents a new genus in the subfamily Bastillevirinae. Arch Virol 165(4):959-962 (DOI:10.1007/s00705-020-04546-1).

Schilling T, Hoppert M, Daniel R, Hertel R (2018) Complete genome sequence of vB_BveP-Goe6, a virus infecting Bacillus velezensis FZB42. Genome Announc 6(8):e00008-18 (DOI:10.1128/genomeA.00008-18).

Nordmann B, Schilling T, Hoppert M, Hertel R (2019) Complete genome sequence of the virus isolate vB_BthM-Goe5 infecting Bacillus thuringiensis. Arch Virol 164(5):1485-1488 (DOI:10.1007/s00705-019-04187-z).

Schilling T, Hoppert M, Hertel R (2018) Genomic analysis of the recent viral isolate vB_BthP-Goe4 reveals increased diversity of φ29-Like Phages. Viruses 10(11):624 (DOI:10.3390/v10110624).

Willms IM, Hoppert M, Hertel R (2017) Characterization of Bacillus subtilis Viruses vB_BsuM-Goe2 and vB_BsuM-Goe3. Viruses 9, 146. (DOI: 10.3390/v9060146)

Willms IM, Hertel R (2016) Phage vB BsuP-Goe1: the smallest identified lytic phage of Bacillus subtilis. FEMS Microbiol Lett 363:fnw208 (DOI: 10.1093/femsle/fnw208 )