CRISPR-Cas9 based phage engineering
This project is a permanent endeavour that has accompanied us since the beginning of our research. It has gone through various phases and led to the development of diverse tools like the programme CutSPR and diverse vectors of which pRH030 is the most relevant.
Status: This project is usually realised within the framework of student work, such as Bachelor and Master Thesis. If you are interested, please contact us.
Otte K, Kühne NM, Furrer AD, Baena Lozada LP, Lutz VT, Schilling T, Hertel R (2020) A CRISPR‐Cas9 tool to explore the genetics of Bacillus subtilis phages. Lett Appl Microbiol 71(6):588-595 (DOI:10.1111/lam.13349).
Schilling T, Dietrich S, Hoppert M, Hertel R (2018) A CRISPR-Cas9-based toolkit for fast and precise in vivo genetic engineering of Bacillus subtilis phages. Viruses 10(5):241 (DOI:10.3390/v10050241).