CutSPR is a program that helps you to plane CRISPR-Cas9 based genomic engineering employing the pJOE8999 vector developed by Josef Altenbuchner, or related plasmids like pRH030. It helps to identify suitable spacer sequences and to create a design recombination cassette for precise modifications.
Compiled versions of the Windows and Linux program and source code can be obtained from the GitHub site of Sascha Dietrich.
Schilling T, Dietrich S, Hoppert M, Hertel R (2018) A CRISPR-Cas9-based toolkit for fast and precise in vivo genetic engineering of Bacillus subtilis phages. Viruses 10(5):241 (DOI:10.3390/v10050241).